beta2-Microglobulin Antibody (Biotin)

• Catalog Number: orb43655
• Category: Antibodies
• Description: Anti-Hu beta2-Microglobulin Biotin
• Clonality: Monoclonal
• Clone Number: B2M-01
• Tested applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Purified human beta2-microglobulin
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: beta2-Microglobulin
• Entrez: 567
• UniProt ID: P61769
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: B2M
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) biotin.

Western blotting analysis of human β2-microglobulin using mouse monoclonal antibody B2M-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse monoclonal antibody followed by IRDye800-conjugated streptavidin. β2-microglobulin was detected around 12 kDa.