beta Amyloid/APP Antibody

• Catalog Number: orb196261
• Category: Antibodies
• Description: beta Amyloid/APP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Bovine, Equine, Monkey, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human APP (672-713aaDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA), different from the related mouse and rat sequences by three amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 86943 MW
• UniProt ID: P05067
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Amyloid beta A4 protein;ABPP;APPI;APP;Alzheimer di
• Note: For research use only
• Application notes: WB: The detection limit for APP is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of APP using anti-APP antibody.Lane 1:human HeLa cell;2:human U-87MG cell;3:human T-47D cell;4:human A549 cell;5:human U2OS cell;6:rat brain tissue;7:mouse brain tissue.

IF analysis of APP using anti-APP antibody.APP was detected in immunocytochemical section of A431 cell.

IHC analysis of APP using anti-APP antibody.APP was detected in paraffin-embedded section of human glioma tissue.

IHC analysis of APP using anti-APP antibody.APP was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of APP using anti-APP antibody.APP was detected in paraffin-embedded section of human renal cancer tissue.

IHC analysis of APP using anti-APP antibody.APP was detected in paraffin-embedded section of human tonsil tissue.

IHC analysis of APP using anti-APP antibody.APP was detected in paraffin-embedded section of rat brain tissue.