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beta-Actin Antibody
SKU: orb69116
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution range | WB: 1/10000 - 1/40000; IP: 1/100-1/200; ICC: 1/50 - 1/500; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Canine, Hamster, Human, Monkey, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone No. | 8H10D10 |
| Immunogen | Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH. |
| Target | beta-Actin |
| Molecular Weight | 42kDa |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
−PS1TP5BP1; ACTB
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Western blot analysis of NIH/3T3 (lane1), Jurkat (lane2), Hela (lane3), CHO (lane4), PC12 (lane5), HEK293 (lane6), COS (lane7), A549 (lane8) and MCF-7 (lane9) cell lysate using beta-Actin antibody.
Flow cytometric analysis of MCF-7 cells negative control (left) using beta Actin antibody (right)
Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin antibody
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
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Chen, Yanbo et al. Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways Artif Cells Nanomed Biotechnol, 47, 2179-2187 (2019)
Applications
WB
Reactivity
Rat
beta-Actin Antibody (orb69116)
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