beta-Actin Antibody

• Catalog Number: orb69116
• Category: Antibodies
• Description: Mouse monoclonal antibody to beta-Actin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8H10D10
• Tested applications: ELISA, FC, ICC, WB
• Reactivity: Hamster, Human, Monkey, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.
• Concentration: 1mg/ml
• Dilution range: WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; RCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 42kDa
• Entrez: 60
• Storage: 4°C; -20°C for long term storage. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Ascitic fluid containing 0.03% sodium azide.
• Alternative names: PS1TP5BP1; ACTB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Chen, Yanbo et al. Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways Artif Cells Nanomed Biotechnol, 47, 2179-2187 (2019)

PubMed: 31159591

Applications

WB

Reactivity

Rat

Western blot analysis of NIH/3T3 (lane1), Jurkat (lane2), Hela (lane3), CHO (lane4), PC12 (lane5), HEK293 (lane6), COS (lane7), A549 (lane8) and MCF-7 (lane9) cell lysate using beta-Actin antibody.

Flow cytometric analysis of MCF-7 cells negative control (left) using beta Actin antibody (right)

Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin antibody