Beta Actin antibody (Biotin)

• Catalog Number: orb420483
• Category: Antibodies
• Description: Beta Actin antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Goat, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rat, Sheep
• Isotype: IgG
• Immunogen: beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 350-375 of Human beta Actin.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This affinity-purified antibody is directed against human beta Actin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
• Conjugation: Biotin
• UniProt ID: P60709
• NCBI: NP_001092.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-beta Actin Antibody biotin conjugation
• Note: For research use only
• Application notes: This affinity-purified antibody has been tested for use in ELISA and is suitable for immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.