Beta-actin Antibody (Ascites)

• Catalog Number: orb2649354
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8H10D10
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:5000~20000, IHC-P: 1:10~50, IHC-P: 1:10~50
• Form/Appearance: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Conjugation: Unconjugated
• MW: 41737 Da
• Target: ACTB recombinant protein is used to produce this monoclonal antibody.
• UniProt ID: P60709
• NCBI: NP_001092.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasm
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Beta-actin Antibody at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 42 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human heart tissue reacted with Beta-actin Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Beta-actin Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-Beta-actin Monoclonal Antibody in HL-60 cell line lysates (35 μg/lane). Beta-actin (arrow) was detected using the purified Mab.