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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:20-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | beclin 1, autophagy related |
| Target | BECN1 |
| Molecular Weight | 60 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunofluorescent analysis of NIH/3T3 cells using Beclin 1 Antibody at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with orb625103 (Beclin 1 Antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human colon cancer using orb625103 (Beclin 1 antibody) at dilution of 1:50.
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Protocol Information
BECN1 Rabbit Polyclonal Antibody (orb625103)
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