Beclin 1/BECN1 Antibody

• Catalog Number: orb196247
• Category: Antibodies
• Description: Beclin 1/BECN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 51896 MW
• UniProt ID: Q14457
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Beclin-1;Coiled-coil myosin-like BCL2-interacting
• Note: For research use only
• Application notes: WB: The detection limit for Beclin 1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-Beclin-1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Beclin 1 using anti-Beclin 1 antibody.Lane 1:human Colo320 cell; 2:human HepG2 cell; 3:human PANC cell; 4:human A431 cell; 5:human Smmc cell; 6:human Jurkat cell; 7:human SW620 cell; 8:human U87 cell.

IF analysis of Beclin-1 using anti-Beclin-1 antibody. Beclin-1 was detected in immunocytochemical section of U20S cells.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of mouse spleen tissue.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of rat intestine tissue.

IHC analysis of Beclin 1 using anti-Beclin 1 antibody. Beclin 1 was detected in a paraffin-embedded section of rat spleen tissue.