BCL2 antibody (PE)

• Catalog Number: orb44690
• Category: Antibodies
• Description: Mouse monoclonal antibody against BCL2 conjugated to PE
• Clonality: Monoclonal
• Clone Number: Bcl-2/100
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Intracellular staining.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: BCL2
• Entrez: 596
• UniProt ID: P10415
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-Apoptosis regulator Bcl 2 antibody, anti- Apo
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (intracellular staining) of human peripheral blood with anti-Bcl2 (Bcl-2/100) PE.

Flow cytometry multicolor staining of human lymphocytes stained using anti-human BCL2 (Bcl-2/100) PE antibody (intracellular staining, 10 µl reagent / 100 µl of peripheral whole blood from DLBCL patient) and anti-human CD19 (LT19) FITC antibody (surface staining, 20 µl reagent / 100 µl of peripheral whole blood from DLBCL patient).

Separation of human BCL-2 positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood from DLBCL patient stained using anti-human BCL2 (Bcl-2/100) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry intracellular staining pattern of human peripheral whole blood from diffuse large B-cell lymphoma (DLBCL) patient. Sample was stained using anti-human BCL2 (Bcl-2/100) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).