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Catalog Number | orb1252066 |
---|---|
Category | Antibodies |
Description | BCL10 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | MUBC10 |
Tested applications | IF, IHC, WB |
Reactivity | Human |
Isotype | IgG1, kappa |
Immunogen | Human recombinant protein was used as the immunogen for this Bcl10 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | IF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the Bcl10 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | BCL10 |
UniProt ID | O95999 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Bcl-10 Antibody: CLAP, mE10, CIPER, c-E10, CARMEN, Read more... |
Note | For research use only |
Application notes | IF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the Bcl10 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Western blot testing of Bcl10 antibody and HepG2 lysate. Observed molecular weight: 26~33 kDa.
IHC staining of human tonsil with Bcl10 antibody (MUBC10).
ELISA, IF, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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