Bcl-XL/BCL2L1 Antibody

• Catalog Number: orb389461
• Category: Antibodies
• Description: Bcl-XL/BCL2L1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Bcl-XL recombinant protein (Position: M1-T219). Human Bcl-XL shares 97.9% amino acid (aa) sequence identity with both mouse and rat Bcl-XL.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26049 MW
• UniProt ID: Q07817
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Bcl-2-like protein 1;Bcl2-L-1;Apoptosis regulator
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-Bcl-XL antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Bcl-XL using anti-Bcl-XL antibody.Lane 1:rat thymus tissue;2:NIH3T3 cell;3:HEPA1-6 cell;4:SGC7901 cell;5:HEPG2 cell.

IHC analysis of Bcl-XL using anti-Bcl-XL antibody.Bcl-XL was detected in paraffin-embedded section of human mammary cancer tissues.

IHC analysis of Bcl-XL using anti-Bcl-XL antibody.Bcl-XL was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of Bcl-XL using anti-Bcl-XL antibody.Bcl-XL was detected in paraffin-embedded section of human tonsil tissues.