Bcl-X Antibody

• Catalog Number: orb2644145
• Category: Antibodies
• Description: Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM519
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: A recombinant human partial protein (amino acids 1-135) was used as the immunogen for this Bcl-X antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Bcl-X antibody is available for research use only.
• UniProt ID: Q07817
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human Hodgkin's lymphoma with Bcl-X antibody (clone SPM519). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Bcl-X antibody (clone SPM519) as confirmation of integrity and purity.