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Bcl-2 Mouse Monoclonal Antibody

SKU: orb499610

Description

Bcl-2 Mouse Monoclonal Antibody

Research Area

Cell Biology

Images & Validation

Tested ApplicationsFC, ICC, WB
Dilution RangeWB=1:1000-5000, ICC/IF=1:100-500, Flow-Cyt=1μg/Test
ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.B3C7
ImmunogenRecombinant human Bcl-2 protein (1-211/239 (C-6x His-Tag)aa)
TargetBCL2
Molecular Weight26 kDa
PurificationAffinity purified by Protein G
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPreservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Bcl-2; PPP1R50; BCL2_HUMAN; BCL2;

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Bcl-2 Mouse Monoclonal Antibody

Sample: Recombinant human Bcl-2 protein Lysate at 40 ug, Primary: Anti-Bcl-2 (orb499610) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26kD, Observed band size: 26kD.

Bcl-2 Mouse Monoclonal Antibody

Sample: U937 (human) cell Lysate at 40 ug, Primary: Anti-Bcl-2 (orb499610) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26kD, Observed band size: 26kD.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol

Bcl-2 Mouse Monoclonal Antibody (orb499610)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
200 μg
$ 870.00
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