Bcl-2-like protein 2 Bcl2L2 Antibody

• Catalog Number: orb137852
• Category: Antibodies
• Description: Bcl-2-like protein 2 Bcl2L2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human BCL2L2 (74-88aa SAQQRFTQVSDELFQ), identical to the related rat and mouse sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By HeatWestern blot, 0.1-0.5μg/ml, Human, Rat, MouseImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 20746 MW
• UniProt ID: Q92843
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Bcl-2-like protein 2;Bcl2-L-2;Apoptosis regulator
• Note: For research use only
• Application notes: WB: The detection limit for BCL2L2 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC analysis of BCL2L2 using anti-BCL2L2 antibody.BCL2L2 was detected in paraffin-embedded section of human rectal cancer tissues.

Western blot analysis of Bcl2L2 using anti-Bcl2L2 antibody.

Flow Cytometry analysis of PC-3 cells using anti-BCL2L2 antibody(Blue line).Isotype control antibody(Green line) was rabbit IgG.Unlabelled sample(Red line) was also used as a control.