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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | FC, ICC, IHC |
|---|---|
| Dilution Range | IHC: 1/200 - 1/1000; IF/ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 8E12H5 |
| Immunogen | Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH. |
| Target | FDA approved drug target related |
| Molecular Weight | 26kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Ascitic fluid containing 0.03% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Bcl-2; BCL2
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Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human brain tissues (right) using BCL-2 antibody
Immunofluorescence analysis of NIH/3T3 cells using BCL-2 antibody
Flow cytometric analysis of 3T3L1 cells using BCL-2 antibo
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Protocol Information
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
BCL-2 Antibody (orb69291)
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