BCKDHA, Phospho (S292) Antibody

• Catalog Number: orb1519848
• Category: Antibodies
• Description: BCKDHA, Phospho (S292) Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: SW-Size, WB
• Reactivity: Human, Mouse
• Immunogen: surrounding serine 292
• Concentration: 40 µg/ml
• Dilution range: WB - 1:1000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BCKDHA, Phospho (S292)
• UniProt ID: P12694
• NCBI: NP_000700.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: BCKDH E1-alpha; OVD1A; MSUD1; MSU; branched chain
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, TCMK-1, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T cells mock treated (-) or treated with phosphatases (+). Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, GaMG, Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-BCKDHA-S292 recombinant monoclonal antibody (orb1519848) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

Detection of mouse pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.