BCA Protein Assay Kit

• Catalog Number: orb219872
• Category: Assays and Kits
• Description: Bicinchoninic Acid Protein Assay Kit
• Reactivity: All
• Application notes: Protein quantification is often required before precessing protein samples for analysis. The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+to Cu1+by proteins in an alkaline environment followed by highly sensitive and selective colorimetric detection of BCA/copper complex. It is water-soluble and strongly absorbs light at 562nm in a linear fashion with increasing protein concentration.
• Range: 15.6 μg/ml - 2000 μg/ml
• Sample Types: All
• Storage: Shipped and store at 4°C for 6 months.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Haiyan Xiang, Yan Wu, Yun Zhang, Yuanhao Hong & Yaling Xu Obtusifolin inhibits podocyte apoptosis by inactivating NF-κB signaling in acute kidney injury Cytotechnology, (2024)

PubMed:

Applications

WB

Reactivity

Mouse

Wei Ji # 1, Guoqing Gong # 2, Yuanhang Liu 1, Yan Liu 1, Jie Zhang 1, Qiang Li Icariin promotes osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by activating PI3K-AKT-UTX/EZH2 signaling in steroid-induced femoral head osteonecrosis J Orthop Surg Res, (2025)

PubMed: 40098175

Representative Standard Curve