BAX (phospho-Ser184) antibody

• Catalog Number: orb4658
• Category: Tools
• Description: BAX (phospho-Ser184) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Bax around the phosphorylation site of Ser184 (TA(p-S)LT)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=3μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: phospho-Bax (Ser184)
• UniProt ID: Q07812
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Bax (phospho S184); Bax (phospho Ser184); p-Bax (S
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

de Vicente, Larissa G. et al. Tlr4 participates in the responses of markers of apoptosis, inflammation, and ER stress to different acute exercise intensities in mice hearts Life Sci., 240, 117107 (2020)

PubMed: 31785241

Paraffin-embedded human lung carcinoma fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (38 degrees for 20 min.). Bax (phospho-Ser184) antibody at 1:200 dilution with overnight incubation at 4