BAX monoclonal antibody (M01), clone 1F5-1B7

• Catalog Number: orb2295582
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant BAX.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: BAX (AAH14175, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTATIFVAGVLTASLTIWKKMG
• Tested applications: ELISA, IF, IHC-P, PLA, WB
• Clone Number: 1F5-1B7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH14175

Immunofluorescence of monoclonal antibody to BAX on HeLa cell. [antibody concentration 10 ug/ml].

Immunoperoxidase of monoclonal antibody to BAX on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml].

Proximity Ligation Analysis of protein-protein interactions between BCL2L1 and BAX. HeLa cells were stained with anti-BCL2L1 rabbit purified polyclonal 1:1200 and anti-BAX mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot detection against Immunogen (46.86 KDa).