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Catalog Number | orb386218 |
---|---|
Category | Antibodies |
Description | This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | KRTH/1576R |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody. |
Dilution range | Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant Basic Cytokeratin antibody is available for research use only. |
UniProt ID | Q01546 |
Storage | Store the recombinant Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human skin using Basic Cytokeratin antibody
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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