BAPTA-AM

SKU: orb1300942

Description

BAPTA-AM is a cell-permeable calcium chelator with high selectivity for Ca²⁺ over Mg²⁺. It is widely used in vitro to investigate calcium-dependent signaling pathways and can also inhibit hERG, hKv1.3, and hKv1.5 potassium channels at micromolar concentrations.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 126150-97-8
• MW: 764.68
• Purity: 99.09%
• Formula: C₃₄H₄₀N₂O₁₈
• SMILES: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccccc1OCCOc1ccccc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O
• Target: Potassium Channel
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:2.5 mg/mL (3.27 mM);DMSO:50 mg/mL (65.39 mM)

Bioactivity

• Target IC50:
  ERG channel (human, HEK293 cells):1.3 μM|Kv1.5 channel (human, HEK293 cells):1.23 μM|Kv1.3 channel (human, HEK293 cells):1.45 μM
• In Vivo:
  METHODS: To investigate the effect on ethanol-induced locomotor activity, BAPTA-AM (0-10 mg/kg, Cremophor EL 1.25% (v/v) in distilled water) was injected intraperitoneally into Swiss (RjOrl) mice, followed by ethanol (0-4 g/kg) 30 min later. RESULTS: Pretreatment with BAPTA-AM blocked the locomotor stimulus produced by ethanol without altering basal locomotion. On the contrary, BAPTA-AM reversed the ethanol-induced hypnosis. METHODS: To investigate the effect on LPS-induced blood-brain barrier leakage, BAPTA-AM (12 mg/kg, 0.01% pluronic acid in sterile saline) was injected intravenously into FVB mice, followed 30 min later by intraperitoneal injection of LPS (25 mg/kg). RESULTS: BAPTA-AM reduced LPS-induced blood-brain barrier leakage.
• In Vitro:
  METHODS: Chondrocytes were treated with BAPTA-AM (10 μM) and FAC (100 μM) for 24 h. Intracellular ROS levels were measured using the Reactive Oxygen Species Assay kit. RESULTS: FAC promoted ROS production and this effect was inhibited by the calcium chelator BAPTA-AM. METHODS: Rat fibroblast RAT2 and Xenopus cells were treated with BAPTA-AM (50 μM) for 1 h, and microtubule depolymerization was detected by Immunostaining. RESULTS: BAPTA AM treatment for 30 min resulted in almost complete disassembly in most cells, and microtubules were uniformly depolymerized in cells within 60 min.
• Cell Research:
  Instructions for use I. Solution preparation 1. Prepare BAPTA-AM storage solution: Dissolve BAPTA-AM in anhydrous DMSO to prepare a 50 mM storage solution; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting). 2. Prepare working solution: Dilute BAPTA-AM storage solution into cell culture medium to a final concentration of 6-50 μM. II. Cell preparation 1. Grow cells to an appropriate density. For adherent cells, they can be planted in culture dishes or culture plates; for suspended cells, adjust the cell concentration to 2×10^6 cells/mL. 2. Incubate cells with BAPTA-AM solution, usually at 37℃, 5% CO₂ for 30-60 minutes, with gentle shaking during the period. 3. Remove excess dye: After incubation, wash cells with calcium-free buffer to remove BAPTA-AM that has not entered the cells. 4. For adherent cells, they can be directly washed with calcium-free PBS; for suspended cells, the cells need to be collected by centrifugation and then resuspended with calcium-free PBS. 5. Detection of changes in calcium ion concentration: You can choose the appropriate detection method according to the experimental requirements: Fluorescence microscope detection: observe the changes in fluorescence intensity in cells. The decrease in fluorescence intensity indicates a decrease in calcium ion concentration. Flow cytometry detection: analyze the changes in fluorescence intensity in cells through flow cytometry. Fluorescence spectrometer detection: measure the changes in fluorescence intensity of cell suspension.

Storage & Handling

• Storage: keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PotassiumChannel, Potassium Channel, BAPTA AM, BAPTA/AM, BAPTAAM, BAPTA-AM, KcsA, inhibit, Inhibitor

Compound Identifiers

PubChem CID

2293