Bag5 Antibody

• Catalog Number: orb18621
• Category: Antibodies
• Description: Bag5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Bag5 recombinant protein (Position: N389-Y447). Human Bag5 shares 96.6% and 93.2% amino acid (aa) sequence identity with mouse and rat Bag5, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 51200 MW
• UniProt ID: Q9UL15
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: BAG family molecular chaperone regulator 5;BAG-5;B
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HL-60 cells using anti-Bag5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of Bag5 using anti-Bag5 antibody.Lane 1:human PC-3 cell; 2:human U-87 cell; 3:human HeLa cell; 4:human K562 cell; 5:rat brain tissue; 6:rat stomach tissue; 7:mouse brain tissue; 8:mouse stomach tissue.

IF analysis of Bag5 using anti-Bag5 antibody. Bag5 was detected in an immunocytochemical section of T-47D cells.