BAG4 antibody

• Catalog Number: orb20057
• Category: Antibodies
• Description: Goat polyclonal antibody to BAG4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:8000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 49.6
• Target: SODD
• Entrez: 9530
• Protein Sequence: GNATSDHPNNQDQSS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti BAG4 antibody, anti BAG-4 antibody, anti SODD
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 60kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6kDa according to NP_004865.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Takada et al, Mol Cell Biol. 2003 Jun;23(11):4026-33.; PMID: 12748303). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb20057 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.