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BAD Antibody
Description
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 1G5B3 |
| Immunogen | Purified recombinant fragment of human BAD (AA: FULL(1-168)) expressed in E. Coli. |
| Target | BAD |
| Molecular Weight | 18.4kDa |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
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ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of MCF-7 cells using BAD antibody

Western blot analysis of human recombinant protein using BAD antibody

Western blot analysis of HEK293 using BAD antibody

Western blot analysis of MCF-7 (Lane1), HEK293 (Lane2) cell lysate using BAD antibody
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Protocol Information
BAD Antibody (orb229798)
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