BACE1 Antibody (N-term)

• Catalog Number: orb1928902
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB16874
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 55764 Da
• Target: This BACE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-70 amino acids from the N-terminal region of human BACE1.
• UniProt ID: P56817
• NCBI: NP_001193978.1, NP_620427.1, NP_036236.1, NP_620429.1, NP_620428.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Beta-secretase 1, Aspartyl protease 2, ASP2, Asp 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-BACE1 Antibody (N-term) in mouse heart tissue lysates (35 ug/lane). BACE1 (arrow) was detected using the purified Pab.

Anti-BACE1 Antibody (N-term) at 1:1000 dilution + human pancreas lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human brain tissue reacted with BACE1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.