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Catalog Number | orb1784569 |
---|---|
Category | Antibodies |
Description | B7-H6 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | ICC, IP, WB |
Reactivity | Human |
Immunogen | residues 25-262 (ECD) |
Concentration | 1000 µg/ml |
Dilution range | WB: 1:1000, IP: 6 µl/mg lysate, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | B7-H6 |
UniProt ID | Q68D85 |
Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
Alternative names | B7 homolog 6; putative Ig-like domain-containing p Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human B7-H6 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, K-562, Hep-G2, Raji, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human B7-H6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569) used for IP at 6 µl/mg lysate
Detection of human B7-H6 by immunocytochemistry. Sample: FFPE section of Hep-G2 cells. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human B7-H6 by immunocytochemistry. Sample: FFPE section of HEK293T cells. Antibody: Rabbit anti-B7-H6 recombinant monoclonal antibody (orb1784569). Secondary: HRP-conjugated goat anti-rabbit IgG.
IHC-P, WB | |
Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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