B7-H3/CD276 Mouse Monoclonal Antibody

• Catalog Number: orb783003
• Category: Antibodies
• Description: B7-H3/CD276 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: F6D12
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,Κ
• Immunogen: Recombinant human CD276.
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-400, IHC-F=1:100-400, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: CD276
• UniProt ID: Q5ZPR3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: B7H3; 4Ig B7 H3; 4Ig-B7-H3; AU016588; B7 H3; B7 ho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of CD276 Antibody in 293T (untransfected) cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemical analysis of CD276 Antibody in MCF-7 cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Sample: Lane 1: 293T-17 cell lysates, Lane 2: A-431 cell lysates, Lane 3: U-20S cell lysates, Lane 4: LNCap cell lysates, Lane 5: Hela cell lysates, Lane 6: MCF-7 cell lysates, Primary: Anti-CD276 (orb783003) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 100 kD.