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Catalog Number | orb1957366 |
---|---|
Category | Proteins |
Description | B7-1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.1 kDa and the accession number is Q00609-1. |
Tag | C-His |
Purity | 98.00% |
MW | 25.1 kDa (predicted); 45-55 kDa (reducing condition, due to glycosylation) |
UniProt ID | Q00609 |
Expression System | HEK293 Cells |
Biological Origin | Mouse |
Biological Activity | B7-1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.1 kDa and the accession number is Q00609-1. |
Expression Region | A DNA sequence encoding the mouse CD80 (Q00609-1) extracellular domain (Met 1-Lys 245) was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Val 38 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation. | |
E.coli |
> 90% as determined by SDS-PAGE | |
50.1 kDa |
> 90% as determined by SDS-PAGE | |
25.8 kDa |
98.00% | |
26.94 kDa (predicted); 56.66 kDa (reducing conditions) |
> 92% by SDS-PAGE. | |
Recombinant Mouse B7-1/CD80 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Val38-Lys245) of mouse B7-1/CD80 (Accession #NP_033985.3) fused with a 6��His Tag at the C-terminus. |