B2M / Beta 2 Microglobulin Antibody (clone B2M-01, Concentrated)

• Catalog Number: orb1539098
• Category: Antibodies
• Description: B2M / Beta 2 Microglobulin Antibody (clone B2M-01, Concentrated)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: B2M-01
• Tested applications: ELISA, FC, IHC, IHC-P, IP, RIA, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Purified human beta2-microglobulin
• Concentration: 1 mg/ml
• Dilution range: ELISA, Flo, IHC, IHC-P (1:50), IP, RIA, WB (2 - 4 µg/ml)
• Conjugation: Unconjugated
• Target: B2M / Beta 2 Microglobulin
• Storage: Store at 2-8°C.
• Buffer/Preservatives: PBS containing 15 mM in sodium azide, pH 7.4. PBS containing 15 mM in sodium azide, pH 7.4
• Alternative names: B2M, Beta 2 Microglobulin, Beta-2-microglobulin, B
• Note: For research use only
• Application notes: Does not react with: Chicken, Bovine, Rabbit, Mouse, Dog. Further information: For use in immunoprecipitation, this antibody coprecipitates b2-microglobulin (12 kDa) and HLA antigens(45 kDa) from the lysates of human lymphoid cells. Recommend U937 human histiocytic lymphoma cell line orRAJI human Burkitt lymphoma cell line as postitive control and EL4 mouse lymphoblastic lymphoma cell line as negative control for Western blot. Reducing and non-reducing conditions. Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Recommend peripheral blood lymphocytes as positve control and DAUDI human Burkitt lymphoma cell line, or erythrocytes as negative control for flow cytometry. Recommend kidney glomeruli as postive control for IHC. For RIA, use at an assay dependent concentration. The dissociation constant of the antibody soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.
• Expiration Date: 12 months from date of receipt.