B Raf (phospho-S365) antibody

• Catalog Number: orb5749
• Category: Antibodies
• Description: B Raf (phospho-S365) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human BRAF around the phosphorylation site of Ser365 (SS(p-S)AP)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 84 kDa
• Target: phospho-BRAF (Ser365)
• UniProt ID: P15056
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: B-Raf (phospho Ser365); B-Raf (phospho S365); B-Ra
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of (line1) rat brain lysates (line2)rat lung lysates using B Raf (phospho-S365) antibody

Paraffin-embedded rat brain tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.).B Raf (phospho-S365) antibody at 1:200 dilution with overnight incubation at 4 degr