B MyB/MYBL2 Antibody

• Catalog Number: orb97054
• Category: Antibodies
• Description: B MyB/MYBL2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human B MyB (1-17aa MSRRTRCEDLDELHYQD), identical to the related mouse sequence and different from the related rat sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 78764 MW
• UniProt ID: P10244
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Myb-related protein B;B-Myb;Myb-like protein 2;MYB
• Note: For research use only
• Application notes: WB: The detection limit for MYBL2 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Confocal immunofluoroscence analysis of A549 cells using MYBL2 antibody

Western blot analysis of Lane 1: Recombinant Human MYBL2 Protein 10ng, Lane 2: Recombinant Human MYBL2 Protein 5ng, Lane 3: Recombinant Human MYBL2 Protein 2.5ng using MYBL2 antibody

Western blot analysis of Lane 1: Rat Spleen Tissue Lysate, Lane 2: Rat Thymus Tissue Lysate, Lane 3: Rat Brain Tissue Lysate, Lane 4: HELA Cell Lysate, Lane 5: COLO320 Cell Lysate, Lane 6: MCF-7 Cell Lysate using MYBL2 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using MYBL2 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Tonsil Tissue using MYBL2 antibody