AZDye647 NT Labeling Kit

SKU: orb2702590

Description

AZDye647 Nick Translation Labeling Kit contains all reagents (except template and materials for purification of the probe) required for nick translation labeling providing a highly efficient, easy-to-perform and rapid labeling technology. \nThe kit is recommended for direct enzymatic labeling of DNA. The AZDye647 NT labeling mix contains specially optimized dUTP-XX-AZDye647 for incorporation into DNA by nick translation using DNA Polymerase I. The excellent stability and quantum yield of the fluorophore combined with a high incorporation rate of the dye-dUTP complex makes it the ideal choice for a broad range of fluorescence applications.\nNick translation labeling is based on the reverse activities of Polymerase I and DNase I. DNase I is able to introduce randomly distributed nicks to double stranded DNA at low enzyme concentrations. The 5'→3' exonuclease activitiy of Polymerase I removes nucleotides from the 3' side of the nick while synthesizing a partial new complementary strand using the 3'-OH termini as primer. In presence of dye-labeled dUTP Polymerase I incorporates labeled dUTP instead of dTTP. The well balanced polymerase / nuclease activities of the enzyme mix ensure the generation of highly labeled double stranded DNA fragments.\nThe resultant DNA is suited for application in FISH, microarray gene expression profiling and other nucleic acid hybridization assays.\nProtect fluorescent labeled dUTP from light and carry out experimental procedures in low light conditions.

Images & Validation

• Application Notes:
  Spectroscopic Properties: λexc 648 nm, λem 671 nm, ε 270.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Storage & Handling

• Storage: store at -20 °C. avoid freeze/thaw cycles, store dark
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Red fluorescent DNA labeling by nick translation

Compound Identifiers

PubChem CID

176419082