Avidin Rhodamine Conjugated

• Catalog Number: orb348736
• Category: Tools
• Description: Avidin Rhodamine Conjugated
• Conjugation: TRITC
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Avidin Rhodamine Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin.
• UniProt ID: P02701
• Tested applications: DOT, FC, FLISA, IF
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: Avidin Rhodamine Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Avidin Rhodamine Conjugated, Avidin TRITC Conjugat
• Research Area: Epigenetics
• Hazard Information: Non-Toxic
• Note: For research use only

Dot Blot of Avidin Rhodamine Conjugated. Antigen: Avidin Rhodamine Conjugated dotted directly to nitrocellulose membrane. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: n/a. Block: orb348637 for 1 HR at RT