Avidin Rhodamine Conjugated

SKU: orb348736

Description

Avidin Rhodamine Conjugated

Research Area

Epigenetics

Images & Validation

• Application Notes:
  Avidin Rhodamine Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. 1.94 moles Rhodamine (TRITC) per mole. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Key Properties

• Purity: Avidin Rhodamine Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin.
• Biological Activity: 1.94
• Conjugation: TRITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Avidin Rhodamine Conjugated, Avidin TRITC Conjugated

Dot Blot of Avidin Rhodamine Conjugated. Antigen: Avidin Rhodamine Conjugated dotted directly to nitrocellulose membrane. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: n/a. Block: orb348637 for 1 HR at RT

Quick Database Links

UniProt

P02701