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Catalog Number | orb348739 |
---|---|
Category | Tools |
Description | Avidin Peroxidase Conjugated |
Tested applications | ELISA, IHC, WB |
Concentration | 2.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000 |
Form/Appearance | Lyophilized |
Purity | Avidin Peroxidase Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin Peroxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Peroxidase. |
Conjugation | HRP |
Hazard Information | Non-Toxic |
UniProt ID | P02701 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Avidin Peroxidase Conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | HRP-Avidin conjugated, Horseradish peroxidase conj Read more... |
Note | For research use only |
Application notes | Avidin Peroxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Peroxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110). |
Expiration Date | 12 months from date of receipt. |
Human | |
0.32-20 nmol/mL | |
0.119 nmol/mL |
Canine | |
31.25-2000 pg/mL | |
12.7 pg/mL |
Human | |
93.75-6000 pg/mL | |
36.7 pg/mL |
Human | |
0.16-10 ng/mL | |
0.053 ng/mL |
Human | |
0.16-10 ng/mL | |
0.062 ng/mL |
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