Avidin Peroxidase Conjugated

SKU: orb348739

Description

Avidin Peroxidase Conjugated

Key Properties

• Purity: Avidin Peroxidase Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin Peroxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Peroxidase.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Avidin Peroxidase Conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 2.0 mg/mL
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

HRP-Avidin conjugated, Horseradish peroxidase conjugated Avidin, AvD, HRP Avidin Biotin Complex, Avidin Peroxidase Conjugated, Avidin HRP Conjugated

Detection of 20S Proteasome Subunits Modified by Reactive Oxygen Species after 2-DE. Images were taken from a study on a differential analysis of ROS sensitive 20S proteasome subunits, which were characterized using biotin-conjugated chemical reagents to visualize the degree of oxidation. A) Free thiol groups of murine 20S proteasome subunits were conjugated with iodoacetyl-LC-Biotin and probed with avidin-HRP. In this indirect assay, diminishing signal indicates an increasing level of oxidation. Parallel detection of free thiol groups in vehicle and ROS treated murine proteasome complexes demonstrated that the α2, β1, β3 and β5i subunits were oxidized at higher levels after ROS treatment. B) In parallel experiments, carbonylation was visualized by derivatization with 2, 4-dinitrophenylhydrazine (DNPH) and probed with an anti-DNP antibody. In this direct assay, the increasing signal indicates higher levels of oxidation. Increased carbonyl modification of 20S proteasome subunits was detected for α2, α4, α6 and β3 after ROS treatment.

Quick Database Links

UniProt

P02701