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Catalog Number | orb348737 |
---|---|
Category | Tools |
Description | Avidin |
Tested applications | ChIP, ELISA, FC, Gel Supershift Assays, SDS-PAGE, WB |
Concentration | 10 mg/mL |
Dilution range | ELISA: User Optimized, ChIP: User Optimized, FC: User Optimized, Gel Supershift Assays: User Optimized, WB: User Optimized |
Form/Appearance | Lyophilized |
Purity | Avidin was prepared from chromatographically purified avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin. No reaction was observed against anti-Streptavidin. |
Conjugation | Unconjugated |
Hazard Information | Non-Toxic |
UniProt ID | P02701 |
Biological Activity | 14.4 U/mg by biotin titration method |
NCBI | CAC34569.1 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Avidin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | Preservative: None. Stabilizer: None. None |
Alternative names | avidin, AVD Read more... |
Note | For research use only |
Application notes | Avidin has been tested by SDS-PAGE and is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized avidin. |
Expiration Date | 12 months from date of receipt. |
Human | |
0.32-20 nmol/mL | |
0.119 nmol/mL |
Canine | |
31.25-2000 pg/mL | |
12.7 pg/mL |
Human | |
93.75-6000 pg/mL | |
36.7 pg/mL |
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