Avidin

SKU: orb348737

Description

Avidin

Research Area

Epigenetics

Images & Validation

• Application Notes:
  Avidin has been tested by SDS-PAGE and is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized avidin.

Key Properties

• Purity: Avidin was prepared from chromatographically purified avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin. No reaction was observed against anti-Streptavidin.
• Biological Activity: 14.4 U/mg by biotin titration method
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Avidin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None. None
• Concentration: 10 mg/mL
• Expiration Date: 12 months from date of receipt.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

avidin, AVD

Average spot thickness as measured with AIR on immobilized PNIPAM-co-AA (gray bar) and PNIPAM-co-AA-biotin (black bar) after consecutive staged incubation with avidin, followed by 10 nM biotinylated anti-human TNF-α (b + TNF-α), and ending with 10 nM TNF-α target detection (TNF-α). Error bars are reported as standard deviations from averaged measurements (n > 3).

In situ generation of AI-2 though surface assembled NF and QS-activated gene expression from ‘docked' bacterial cells. (B) NF targeting and subsequent in vitro AI-2 synthesis on avidin plate wells. NF loading was determined by FITC fluorescence and AI-2 concentrations were measured by Ellman's assay. (C) E. coli W3110 (ΔlsrFG ΔluxS) response to in vitro AI-2 synthesized by NF assembled onto avidin plate wells. Bacterial suspensions were added directly to wells and DsRed intensities were measured via fluorescence plate reader. The negative control is without SAH addition.

SDS-Page of Avidin. Lane 1: Avidin - reduced. Lane 2: Avidin - non-reduced. Load: 1.0 µg per lane. Predicted/Observed size: 16 kDa for Avidin. Other Band(s): None.

Quick Database Links

UniProt

P02701

RefSeq:CAC34569.1