Avidin Fluorescein Conjugated

• Catalog Number: orb348738
• Category: Tools
• Description: Avidin Fluorescein Conjugated
• Conjugation: FITC
• Form/Appearance: Lyophilized
• Concentration: 2.2 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Avidin Fluorescein Conjugated was prepared from chromatographical pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Avidin. No reaction was observed against anti-Streptavidin.
• UniProt ID: P02701
• Tested applications: DOT, FC, FLISA, IF
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: Avidin Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.
• Biological Activity: 3.55
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Avidin Fluorescein Conjugated, Avidin FITC Conjuga
• Research Area: Epigenetics
• Hazard Information: Non-Toxic
• Note: For research use only

BSA-FITC solution exposed to three time-averaged patterns simultaneously at 110 mW for 10 s. (a) Real-time microscope image captured during fabrication. (b) Fluorescent image of patterned protein after sample wash with 1x PBS. (c) SEM image of sample after a chemical desiccation process. Predicted feature dimensions were assessed using area measurements from both the fluorescent and SEM images. The fluorescent measurements indicated the features were ~6% larger than desired, while the SEM measurements indicated they were ~6% smaller than desired; demonstrating an accurate tolerance for pattern transfer. Next, a BSA-FITC solution spiked with avidin conjugated FITC was patterned. Biotin conjugated with ATTO 655 was applied to the resulting BSA/avidin structures for 20 min to assess the retention of avidin binding ability. Fluorescent microscope images were taken using (d) FITC and (e) Cy5 filter sets. A combined fluorescent image (f) reveals the functionality of the substrate after patterning. All scale bars are 50 µm.

Dot Blot of Avidin Fluorescein conjugated. Lane A: BSA Biotin Conjugated. Lane B: BSA. Lanes 1 load: 100 ng, 2-5: serial dilution 3 fold. Primary Antibody: n/a. Secondary Antibody: Avidin Fluorescein Conjugated at 1 µg/ml at RT for 1 hour. Block: orb348637 for 30 min at RT.