Avidin antibody (Biotin)

• Catalog Number: orb344992
• Category: Antibodies
• Description: Avidin antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Avidin (Hen Egg White)
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:250 - 1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: Anti-Avidin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, as well as purified and partially purified Avidin [Hen Egg White].
• Conjugation: Biotin
• UniProt ID: P02701
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Avidin Antibody biotin Conjugation, Bi
• Note: For research use only
• Application notes: Anti-Avidin Antibody Biotin Conjugated has been tested by ELISA and western blot and is suitable for immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Avidin. Lane 2: None. Load: 100 ng per lane using Avidin antibody (Biotin)