AURKA antibody

• Catalog Number: orb18316
• Category: Antibodies
• Description: Goat polyclonal antibody to AURKA
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 45.8
• Target: Aurora kinase A / AURKA
• Entrez: 6790
• Protein Sequence: QNKESASKQS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti AURKA antibody, anti aurora kinase A antibody
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 45kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8kDa according to NP_003591.2). In transfected HEK293 transiently expressing AURKA a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18316 (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Aurora kinase A (RC212018) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with orb18316 in the right panel (vector-only transfection in first and fourth lanes).