ATXN3 Antibody (Center)

• Catalog Number: orb1928026
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23951
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 41250 Da
• Target: This ATXN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-288 amino acids from the Central region of human ATXN3.
• UniProt ID: P54252
• NCBI: NP_109376.1, NP_001158252.1, NP_001121168.1, NP_004984.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ataxin-3, Machado-Joseph disease protein 1, Spinoc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATXN3 Antibody (Center) in MCF-7 cell line lysates (35 ug/lane). ATXN3 (arrow) was detected using the purified Pab.

Western blot analysis of ATXN3 (arrow) using rabbit polyclonal ATXN3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATXN3 gene.

ATXN3 Antibody (Center) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ATXN3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.