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Catalog Number | orb308778 |
---|---|
Category | Antibodies |
Description | ATX2/ATXN2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ATX2 (1283-1313aa QSALQPIPVSTTAHFPYMTHPSVQAHHQQQL), identical to the related mouse sequence. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 140283 MW |
UniProt ID | Q99700 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Ataxin-2;Spinocerebellar ataxia type 2 protein;Tri Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody.Lane 1:human HeLa cell; 2:human K562 cell; 3:human A549 cell; 4:rat brain tissue; 5:mouse brain tissue; 6:mouse liver tissue.
IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody. ATX2/ATXN2 was detected in a paraffin-embedded section of human breast cancer tissue.
IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody. ATX2/ATXN2 was detected in a paraffin-embedded section of human colonic adenoma cancer tissue.
IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody. ATX2/ATXN2 was detected in a paraffin-embedded section of human glioma tissue.
IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody. ATX2/ATXN2 was detected in a paraffin-embedded section of human placenta tissue.
ELISA, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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