ATP6V1E1 antibody

• Catalog Number: orb412202
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATP6V1E1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human ATP6V1E1
• Dilution range: WB: 1:500-2000, IP: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ATP6V1E1
• Entrez: 297566, 529, 11973
• UniProt ID: P36543, P50518, Q6PCU2
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-ATP6E antibody, anti-ATP6E2 antibody, anti-V-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATP6V1E1 expression in LO2 (A), mouse liver (B), rat brain (C) whole cell lysates. (Predicted band size: 22; 23; 26 kD; Observed band size: 31 kD)

Immunofluorescent analysis of ATP6V1E1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).