ATP5G1,2,3/ATP5MC1,2,3 Antibody

• Catalog Number: orb546394
• Category: Antibodies
• Description: ATP5G1,2,3/ATP5MC1,2,3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ATP5F1/ATP5MC1 recombinant protein (Position: D62-L113).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunofluorescence, 2μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 10-14 kDa
• UniProt ID: P05496
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: ATP synthase F (0) complex subunit C1,2,3, mitocho
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HL-60 cells using anti-ATP5G1, 2, 3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of PC-3 cells using anti-ATP5G1, 2, 3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB:Lane 1:HEPG2;2:A549;3:PC-3;4:HL-60;5:HEK293 cell;6:U937 cell;7:CACO-2 cell;8:rat kidney tissue;9:rat stomach tissue;10:rat liver tissue;11:mouse kidney tissue;12:mouse stomach tissue;13:mouse liver tissue;14:mouse lung tissue;15:Neuro-2a cell.

IF analysis of ATP5G1, 2, 3 using anti-ATP5G1, 2, 3 antibody. ATP5G1, 2, 3 was detected in paraffin-embedded section of human intestinal tissue.

IHC analysis of ATP5G1, 2, 3 using anti-ATP5G1, 2, 3 antibody. ATP5G1, 2, 3 was detected in paraffin-embedded section of rat intestine tissues.

IHC analysis of ATP5G1, 2, 3 using anti-ATP5G1, 2, 3 antibody.ATP5G1, 2, 3 was detected in paraffin-embedded section of human Ovarian cancer tissues.

IHC analysis of ATP5G1, 2, 3 using anti-ATP5G1, 2, 3 antibody.ATP5G1, 2, 3 was detected in paraffin-embedded section of human tonsil tissues.

IHC analysis of ATP5G1, 2, 3 using anti-ATP5G1, 2, 3 antibody.ATP5G1, 2, 3 was detected in paraffin-embedded section of human glioma tissues.

IHC analysis of ATP5G1, 2, 3 using anti-ATP5G1, 2, 3 antibody.ATP5G1, 2, 3 was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of ATP5G1, 2, 3 using anti-ATP5G1, 2, 3 antibody.ATP5G1, 2, 3 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of ATP5G1, 2, 3 using anti-ATP5G1, 2, 3 antibody.ATP5G1, 2, 3 was detected in paraffin-embedded section of mouse intestine tissues.