ATP5F1 Antibody

• Catalog Number: orb1248196
• Category: Antibodies
• Description: ATP5F1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SIQHIQNAIDTE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25kDa band observed in Human Heart lysates (calculated MW of 28.9kDa according to NP_001679.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 25kDa
• Target: ATP5F1
• UniProt ID: P24539
• NCBI: NP_001679.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ATP5F1, ATP synthase, H+ transporting, mitochondri
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25kDa band observed in Human Heart lysates (calculated MW of 28.9kDa according to NP_001679.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248196 (0.03 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248196 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.