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Catalog Number | orb2295688 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant ATP2A1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1B11 |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | ATP2A1 (NP_775293, 522 a.a. ~ 612 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | IDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQ |
NCBI | NP_775293 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ATP2A1 monoclonal antibody (M01), clone 1B11. Western Blot analysis of ATP2A1 expression in A-431.
Detection limit for recombinant GST tagged ATP2A1 is 0.03 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to ATP2A1 on A-431 cell. [antibody concentration 10 ug/ml].
Immunoperoxidase of monoclonal antibody to ATP2A1 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml].
Western Blot analysis of ATP2A1 expression in transfected 293T cell line by ATP2A1 monoclonal antibody (M01), clone 1B11. Lane 1: ATP2A1 transfected lysate(109.3 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (35.75 KDa).