ATP1B1 antibody

• Catalog Number: orb178604
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATP1B1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 63-303 of human ATP1B1 (NP_001668.1).
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Conjugation: Unconjugated
• MW: 55kDa
• UniProt ID: P05026
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Alternative names: anti ATP1B antibody, anti ATP1B1 antibody, anti Be
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of C6 cells using ATP1B1 Rabbit pAb (orb178604) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ATP1B1 Rabbit pAb (orb178604) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ATP1B1 Rabbit pAb (orb178604) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using ATP1B1 Rabbit pAb (orb178604) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse stomach using ATP1B1 Rabbit pAb (orb178604) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western blot analysis of various lysates using ATP1B1 Rabbit pAb (orb178604) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western blot analysis of various lysates using ATP1B1 Rabbit pAb (orb178604) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.