ATK/BTK Rabbit Polyclonal Antibody

SKU: orb5650

Description

ATK/BTK Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC
• Dilution Range: ICC/IF=1:50-200, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BTK (581-659/659aa)
• Target: BTK
• Molecular Weight: 76 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AGMX1; AT; ATK; BPK; IGHD3; IMD1; PSCTK1; XLA; xid; BTK_HUMAN; BTK; Agammaglobulinemia tyrosine kinase (ATK); B-cell progenitor kinase (BPK); Bruton tyrosine kinase; 2.7.10.2; BTK_MOUSE; Kinase EMB;

Blank control (black line): Raw264.7. Primary Antibody (green line): Rabbit Anti-ATK/BTK antibody (orb5650), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 11: Human Raji cell lysates, Lane 12: Human K562 cell lysates, Lane 13: Human Ramos cell lysates, Primary: Anti-ATK/BTK (orb5650) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 76 kDa, Observed band size: 77 kDa.

Quick Database Links

Gene Symbol

BTK

UniProt

Q06187