ATG4A Antibody

• Catalog Number: orb1732274
• Category: Antibodies
• Description: The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide of human ATG4A
• Concentration: 1mg/ml
• Dilution range: IP: 1/20; WB: 1/500-1/1000; IHC: 1/50-1/100
• Conjugation: Unconjugated
• MW: Calculated MW: 45 kDa; Observed MW: 45 kDa
• Entrez: 115201
• UniProt ID: Q8WYN0
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: ATG4A; APG4A; AUTL2; Cysteine protease ATG4A; AUT-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.