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    ATG3 antibody

    Catalog Number: orb340975

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb340975
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to ATG3
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenRecombinant full length protein of human ATG3
    Dilution rangeWB: 1:500-1:2000, IHC-P: 1:50-1:200
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetATG3
    Entrez171415, 67841, 64422
    UniProt IDQ9NT62, Q6AZ50, Q9CPX6
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesAPG3; APG3L; Ubiquitin-like-conjugating enzyme ATG
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    ATG3 antibody

    Western blot analysis of ATG3 expression in Raji (A), Hela (B), mouse liver (C), mouse heart (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 40 kD)

    ATG3 antibody

    Immunofluorescent analysis of ATG3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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