ATF5 Rabbit Monoclonal Antibody

• Catalog Number: orb548102
• Category: Antibodies
• Description: ATF5 Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: HDD-1
• Tested applications: ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human ATF5
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Dilution range: WB 1:1000-1:2000IHC 1:100-1:500ICC/IF 1:50-1:200IP 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 30674 MW
• UniProt ID: Q9Y2D1
• Sensitivity: > 5000 cells
• Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Alternative names: Cyclic AMP-dependent transcription factor ATF-5;cA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATF5 expression in (1) Jurkat cell lysate; (2) 3T3 cell lysate; (2) C6 cell lysate.

Immunohistochemical analysis of paraffin-embedded Human breast, using ATF5 Antibody.

ICC staining of ATF5 in HeLa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS.