ATF4 antibody

• Catalog Number: orb213589
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATF4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATF4. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ATF4
• Entrez: 79255, 468, 11911
• UniProt ID: P18848, Q9ES19, Q06507
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti CREB2 antibody, anti TXREB antibody, anti Cyc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATF4 expression in SGC7901 (A), C6 (B), EC7901 (C) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 55 kD)

Immunohistochemical analysis of ATF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.