ATF1 monoclonal antibody (M03), clone 3E7

• Catalog Number: orb2295749
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full-length recombinant ATF1.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1 Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: ATF1 (AAH29619, 1 a.a. ~ 271 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV
• Tested applications: ELISA, IHC-P, WB
• Clone Number: 3E7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH29619

Detection limit for recombinant GST tagged ATF1 is 0.3 ng/ml as a capture antibody.

Immunoperoxidase of monoclonal antibody to ATF1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml].

Western Blot analysis of ATF1 expression in transfected 293T cell line by ATF1 monoclonal antibody (M03), clone 3E7. Lane 1: ATF1 transfected lysate(29.2 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of ATF1 over-expressed 293 cell line, cotransfected with ATF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ATF1 monoclonal antibody (M03), clone 3E7 (Cat # orb2295749). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (55.55 KDa).